Chitosan-induced production of secondary metabolites in plant extracts of Piper auritum, and the in vitro fungicidal activity against Fusarium oxysporum f. sp. vanillae

María del Socorro Fernández, Flavio Hernández-Ochoa, Oscar Carmona-Hernández, Mauricio Luna-Rodríguez, Carolina Barrientos-Salcedo, Hugo Asselin, José Armando Lozada-García

Abstract


The effect of chitosan addition on the production of secondary metabolites of Piper auritum ethanolic extracts was tested, as well as the in vitro fungicidal activity against Fusarium oxysporum f. sp. vanillae. Piper auritum plants were divided into six parcels and commercial chitosan was added to half of them. The concentrations of flavonoids, phenols, terpenes, alkaloids and salicylic acid were measured in P. auritum ethanolic extracts and the antifungal activity was measured with the median effective concentration (EC50). The concentrations of total flavonoids, phenols and terpenes were higher with chitosan treatment (respectively 12.8 vs 12.4 ?g quercetin equivalent per mg, 12.6 vs. 2.3 ?g tannic acid equivalent per 10 mg, and 16.3 vs. 11.6 mg menthol equivalent per 100 mg). However, the alkaloid concentration was reduced by chitosan addition (from 148.2 to 84.5 ?g piperine equivalent per mg). Chitosan addition increased the concentration of salicylic acid (from 1.3 to 2.2 ?g salicylic acid equivalent per mg). A 4 mg mL-1 ethanolic extract of P. auritum treated with chitosan inhibited 100% of mycelial growth. The EC50 of P. auritum against F. oxysporum f. sp. vanillae was lower with chitosan treatment (1.5 mg mL-1) compared to control (5.1 mg mL-1). Chitosan addition increased secondary metabolite production and in vitro antifungal activity in P. auritum extracts.

Keywords


ethanolic extracts; Mexican pepperleaf; hoja santa

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References


Ádám AL, Nagy ZÁ, Kátay G, Mergenthaler E and Viczián O. 2018. Signals of systemic immunity in plants: Progress and open questions. International Journal of Molecular Sciences 19(4): 1146. https://doi.org/10.3390/ijms19041146

Amini J and Sidovich D. 2010. The effects of fungicides on Fusarium oxysporum f. sp. lycopersici associated with Fusarium wilt of tomato. Journal of Plant Protection Research 50: 172?178. https://doi.org/10.2478/v10045-010-0029-x

Báez-Valdez EP, Carrillo-Fasio JA, Báez-Sañudo MA, García-Estrada RS, Valdez-Torres JB and Contreras-Martínez R. 2010. Uso de portainjertos resistentes para el control de la fusariosis (Fusarium oxysporum f. sp. lycopersici Snyder & Hansen raza 3) del tomate (Lycopersicon esculentum Mill) en condiciones de malla sombra. Revista Mexicana de Fitopatología 28: 111?123. http://www.scielo.org.mx/pdf/rmfi/v28n2/v28n2a4.pdf

Benhamou N and Thériault G. 1992. Treatment with chitosan enhances resistance of tomato plants to the crown and root rot pathogen Fusarium oxysporum f. sp. lycopersici. Physiological and Molecular Plant Pathology 41: 33?52. https://doi.org/10.1016/0885-5765(92)90047-Y

Blainski A, Lopes GC and De Mello JCP. 2013. Application and analysis of the Folin Ciocalteu method for the determination of the total phenolic content from Limonium brasiliense L. Molecules 18: 6852?6865. https://doi.org/10.3390/molecules18066852

Brasili E, Pratico G, Marini F, Valletta A, Capuani G, Sciubba F, Miccheli A and Pasqua G. 2014. A non-targeted metabolomics approach to evaluate the effects of biomass growth and chitosan elicitation on primary and secondary metabolism of Hypericum perforatum in vitro roots. Metabolomics 10: 1186?1196. https://doi.org/10.1007/s11306-014-0660-z

Carmona-Hernández O, Fernández MS, Palmeros-Sánchez B and Lozada-García JA. 2014. Actividad insecticida de extractos etanólicos foliares de nueve piperaceas (Piper spp.) en Drosophila melanogaster. Revista Internacional de Contaminación Ambiental 30: 67?73. https://www.redalyc.org/pdf/370/37033725008.pdf

Chang JH, Shin JH, Chung IS and Lee HJ. 1998. Improved menthol production from chitosan-elicited suspension culture of Mentha piperita. Biotechnology Letters 20: 1097?1099. https://doi.org/10.1023/A:1005396924568

da Luz SFM, Yamaguchi LF, Kato MJ, de Lemos OF, Xavier LP, Maia JGS, Ramos AR, Setzer WN and da Silva JKR. 2017. Secondary metabolic profiles of two cultivars of Piper nigrum (black pepper) resulting from infection by Fusarium solani f. sp. piperis. International Journal of Molecular Sciences 18: 2434. https://doi.org/10.3390/ijms18122434

Dweba CC, Figlan S, Shimelis HA, Motaung TE, Sydenham S, Mwadzingeni L and Tsilo TJ. 2017. Fusarium head blight of wheat: Pathogenesis and control strategies. Crop Protection 91: 114?122. https://doi.org/10.1016/j.cropro.2016.10.002

Erisléia-Meireles N, Luciana-Xavier P, Alessandra-Ramos R, José-Guilherme MS, William-Setzer N and da Silva JRK. 2016. Phenylpropanoids produced by Piper divaricatum, a resistant species to infection by Fusarium solani f. sp. piperis, the pathogenic agent of fusariosis in black pepper. Journal of Plant Pathology and Microbiology 7: 333. https://doi.org/10.4172/2157-7471.1000333

Ghorai N, Ghorai N, Chakraborty S, Gucchait S, Saha SK and Biswas S. 2012. Estimation of total terpenoids concentration in plant tissues using a monoterpene, Linalool as standard reagent. Protocol Exchange. November. https://dx.doi.org/10.1038/protex.2012.055

Golkar P, Taghizadeh M and Yousefian Z. 2019. The effects of chitosan and salicylic acid on elicitation of secondary metabolites and antioxidant activity of safflower under in vitro salinity stress. Plant Cell, Tissue and Organ Culture 137(3): 575?585.

López-Moya F, Suarez-Fernández M and López-Llorca LV. 2019. Molecular mechanisms of chitosan interactions with fungi and plants. International Journal of Molecular Sciences 20: 332. https://doi.org/10.3390/ijms20020332

McGovern RJ. 2015. Management of tomato diseases caused by Fusarium oxysporum. Crop Protection 73: 78?92. https://doi.org/10.1016/j.cropro.2015.02.021

Nascimento SB, de Mattos C, Cezar J, de Menezes IC, Reis Duarte MDL, Darnet S, Harada ML and de Souza CRB. 2009. Identifying sequences potentially related to resistance response of Piper tuberculatum to Fusarium solani f. sp. piperis by suppression subtractive hybridization. Protein and Peptide Letters 16: 1429?1434. https://doi.org/10.2174/092986609789839368

Pineda RM, Vizcaíno SP, García CM, Gil JH, Durango DL. 2012. Chemical composition and antifungal activity of Piper auritum Kunth and Piper holtonii C. DC. against phytopathogenic fungi. Chilean Journal of Agricultural Research 72: 507?515. http://www.chileanjar.cl/files/V72I4Y2012CJAR120090.pdf

Pitta-Alvarez SI and Giulietti AM. 1999. Influence of chitosan, acetic acid and citric acid on growth and tropane alkaloid production in transformed roots of Brugmansia candida. Effect of medium pH and growth phase. Plant Cell, Tissue and Organ Culture 59: 31?38. https://doi.org/10.1023/A:1006359429830

Potzernheim MCL, Bizzo HR, Silva JP and Vieira RF. 2012. Chemical characterization of essential oil constituents of four populations of Piper aduncum L. from Distrito Federal, Brazil. Biochemical Systematics and Ecology 42: 25?31. https://doi.org/10.1016/j.bse.2011.12.025

Rahman A, Sultana V, Ara J and Ehteshamul-Haque S. 2016. Induction of systemic resistance in cotton by the neem cake and Pseudomonas aeruginosa under salinity stress and Macrophomina phaseolina infection. Pakistan Journal of Botany 48: 1681?1689. http://www.pakbs.org/pjbot/PDFs/48(4)/45.pdf

Rongai D, Pulcini P, Pesce B and Milano F. 2015. Antifungal activity of some botanical extracts on Fusarium oxysporum. Open Life Science 10: 409?416. https://doi.org/10.1515/biol-2015-0040Scott IM, Jensen HR,

Philogène BJ and Arnason JT. 2008. A review of Piper spp. (Piperaceae) phytochemistry, insecticidal activity and mode of action. Phytochemistry Reviews 7: 65?75. https://doi.org/10.1007/s11101-006-9058-5

Suprapta DN and Ohsawa K. 2007. Fungicidal activity of Piper betle extract against Fusarium oxysporum f. sp. vanillae. Journal of the International Society for Southeast Asian Agricultural Sciences 13: 40?46. http://issaasphil.org/wpcontent/uploads/2020/02/J-Issaas-v13n2-December-2007-Full-Journal.pdf

Tiwari RK, Udayabhanu M. and Chanda S 2016. Quantitative analysis of secondary metabolites in aqueous extract of Clerodendrum serratum. International Research Journal of Pharmacy 7: 61?65. https://doi.org/10.7897/2230-8407.0712148

Wiesel L, Newton AC, Elliott I, Booty D, Gilroy EM, Birch PR and Hein I. 2014. Molecular effects of resistance elicitors from biological origin and their potential for crop protection. Frontiers in Plant Science 5: 1-13. https://doi.org/10.3389/fpls.2014.00655

Yin H, Frette? XC, Christensen LP and Grevsen K. 2012. Chitosan oligosaccharides promote the content of polyphenols in Greek oregano (Origanum vulgare ssp. hirtum) Journal of Agricultural and Food Chemistry 60(1): 136?143. https://doi.org/10.1021/jf204376j




DOI: http://dx.doi.org/10.18781/R.MEX.FIT.2006-6

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