Etiology of wilt and basal rot of Jatropha curcas in Arriaga, Chiapas, Mexico

Wester Moises Salzar-Pinacho, Ricardo René Quiroga-Madrigal, María de los Ángeles Rosales-Esquinca, Eduardo Raymundo Garrido-Ramírez, Fransisco Holguín-Meléndez, Primera publicación / First published: 26 de noviembre, 2015, DOI: 10.18781/R.MEX.FIT.1507-5

Abstract


In the municipality of Arriaga, Chiapas, the death of physic nut (Jatropha curcas L.) 1 and 2-year old plantations has been observed since 2009, with symptoms of wilting and basal stem rot. The registered incidence has been up to 85%; the virulence of the pathogen is very high because it causes the death of the plant in a period of 20 to 30 days. The objective of this study was to determine the causal agent of the physic nut death. Laboratory isolates were conducted from field samples of diseased plant material of stem and root tissue, in Petri dishes with B King (BK) and triphenyl tetrazolium chloride (TTC) medium, incubated at 28 °C for 48 h. The isolates obtained were purified and subjected to biochemical and hypersensitivity tests on tobacco (Nicotiana tabacum) cv. Xanthi. The identification was positive for three isolates of Ralstonia solanacearum. However, it was not possible to complete Koch's postulates; therefore, R. solanacearum is only consigned at association level.

Keywords


Physic nut; Ralstonia solanacearum; diagnosis; biochemical tests

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DOI: http://dx.doi.org/10.18781/R.MEX.FIT.1507-5

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