Search for sources of resistance to Poinsettia mosaic virus in wild poinsettia plants

Omar Jacobo-Villegas, Guadalupe Valdovinos-Ponce, Sergio Ramírez-Rojas, Camilo Hernández-Juárez

Abstract


Poinsettia, as an improved species, is one of the most economically important ornamental plants worldwide. In 2013, the value of poinsettia production in Mexico was higher than 416 million pesos; however, this amount can be reduced by PnMV, which has been reported in some countries of the Americas, Asia and Europe inducing symptoms that affect its marketing. Taking into account that Mexico imports improved varieties of poinsettia for production and marketing, and that these varieties were developed originally from wild plants collected by Robert Poinsett in Mexico and sent them to USA, the objective of this research was to search for a virus resistant source in wild poinsettia plants. Symptomless wild poinsettia plants and plants with symptoms associated with virus were collected in four states of Mexico. DAS-ELISA and RT-PCR were done in order to determine the virus presence as well as mechanical and graft inoculations. Results showed that the wild poinsettia plants are non-hosts for PnMV, so they may be a source of resistance to this virus. This is the first report of PnMV in improved poinsettia plants in Mexico. 


Keywords


Euphorbia pulcherrima; resistance; virus

Full Text:

PDF (Español)

References


Agrios GN. 2005. Plant Pathology. Elsevier Academic Press. New York. 922 p.

aus dem Siepen M, Pohl JO, Koo BJ, Wege C and Jeske H. 2005. Poinsettia latent virus is not a cryptic virus, but a natural poleovirus-sobemovirus hybrid. Virology 336: 240-250.

Bech K. 1996. Methods to eliminate Poinsettia mosaic virus in Euphorbia pulcherrima and reinfection by different methods to reveal the nature of the branching factor. International Society for Horticultural Science (ISHS). Phytoparasitica 24: 346.

Canul KJ, García PF, Ramírez RS, Osuna CFJ. 2010. Programa de mejoramiento genético de nochebuena en Morelos. Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación (SAGARPA). Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias (INIFAP). México. Publicación especial No. 49. 36 p.

Canul KJ, García PF, Barrios GEJ, Osuna CFJ. Ramírez RS, Alia TI y Montoya CRE. 2014. Caracterización morfológica de nochebuena (Euphorbia pulcherrima Willd. ex Klotzsch). Ciencia y Tecnología Agropecuaria de México 2:16-23.

Carballo O, Izaguirre ML, and Marys E. 2001. Detection of Poinsettia mosaic virus Infecting Poinsettias (Euphorbia pulcherrima) in Venezuela. Plant Disease 85: 1208.

Colinas LMT, Mejía MJM, Espinoza FA, Alía TI, Martínez MF, Rodríguez EF y Flores EC. 2009. La Nochebuena de sol o de jardín. Folleto técnico. SNICS, SINAREFI, UACH. 15 p.

Chung BN, Lee EK, Jeong MI and Kim HR. 2004. First Report of Poinsettia mosaic virus in Korea. The Plant Pathology Journal 20: 220-223.

Galindo GDB, Alia TI, Andrade RM, Colinas LMT, Canul KJ, Sainz AMJ. 2012. Producción de nochebuena de sol en Morelos, México. Revista Mexicana de Ciencias Agrícolas 3: 751-763.

García ROG, Pérez ML, Navarro LMJ, Salas AMD, Martínez JOA, León GMF y Núñez PHG. 2014. Virus fitopatógenos en insectos asociados al ajo. Nota científica. Revista Chapingo. Serie Horticultura 20: 147-156.

Jayasinghe U y Chuquillanqui C. 1992. Uso de plantas indicadoras para la detección de virus en papa. Guía de Investigación CIP21. Lima, Perú. 30 p.

Lebas BSM, Ochoa CFM, Elliott DR, Tang JZ and Alexander BJR. 2007. Detection of Poinsettia mosaic virus by RT-PCR in Euphorbia spp. in New Zealand. Plant Disease 91:110.

Martins DR, Rodrigues SE, Pedroso J Arakava R, Rodrigues A, Lazarini B and Batista V. 2008. Detection and Identification of TMV infecting tomato under protected cultivation in Paraná state. Brazilian archives of Biology and technology 51:903-909.

Ocampo OT. 2012. Identificación de virus asociados a la nochebuena de sol (Euphorbia pulcherrima Willd. Ex Klotzch) en México. Tesis de Maestría. Colegio de Postgraduados. Montecillo, México. 47 p.

Ocampo OT, Ochoa MDL, Ramírez RS, Valdovinos PG y Nava DC. 2013. Primer reporte de Tobacco mosaic virus (TMV) y Poinsettia mosaic virus (PnMV) en nochebuena de sol (Euphorbia pulcherrima Willd. Ex Klotzsch) en México. Revista Internacional de Botánica Experimental 82: 235-241.

SAGARPA, Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación. 2014. Servicio de información agroalimentaria y pesquera.www.siap.gob.mx/cierre-de-la-produccion-agricola-por-cultivo (consulta, mayo 2015).

SINAREFI. 2010. Red Nochebuena. Diagnóstico. Sistema Nacional de Recursos Fitogenéticos para la Alimentación y la Agricultura (SINAREFI). SAGARPA. 124 p.

Spetz C, Moe R and Blystad DR. 2008. Symptomless infectious cDNA clone of a Norwegian isolate of Poinsettia mosaic virus. Archives of virology 153: 1347-1351.

Trejo L, Feria ATP, Olsen KM, Eguiarte LE, Arroyo B, Gruhn JA and Olson ME. 2012. Poinsettia’s wild ancestor in the Mexican dry tropics: historical, genetic, and environmental evidence. American Journal of Botany 99: 1146–1157.

USDA. 2104. Floriculture Crops 2013 Summary (June 2014). USDA, National Agricultural Statistics Service. 59 p.

Zamboni A, Pierantoni L, De Franceschi P. 2008. Total RNA extraction from strawberry tree (Arbutus unedo) and several other woody-plants. iForest 1: 122.125.


Refbacks

  • There are currently no refbacks.